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sandwich enzyme linked immuno sorbant assay elisa  (TaKaRa)


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    Structured Review

    TaKaRa sandwich enzyme linked immuno sorbant assay elisa
    Sandwich Enzyme Linked Immuno Sorbant Assay Elisa, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 213 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/procollagen+type+i+c+peptide/Procollagen+Type+I+C-Peptide+(PIP)+EIA+Kit/us12605328-545-18-25
    Average 96 stars, based on 213 article reviews
    sandwich enzyme linked immuno sorbant assay elisa - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    other:

    Article Title: The Impact of Substrate Stiffness and Mechanical Loading on Fibroblast-Induced Scaffold Remodeling
    Article Snippet: Fibroblasts as many other cells are known to form, contract, and remodel the extracellular matrix (ECM).. The presented study aims to gain an insight into how mechanical boundary conditions affect the production of ECM components, their remodeling, and the feedback of the altered mechanical cell environment on these processes.. The influence of cyclic mechanical loading (f = 1 Hz, 10% axial compression) and scaffold stiffness (E = 1.2 and 8.5 kPa) on the mechanical properties of fibroblast-seeded scaffold constructs were investigated in an in vitro approach over 14 days of culture.

    Article Title: A small-molecule TNIK inhibitor targets fibrosis in preclinical and clinical models.
    Article Snippet: INS018_055 was provided by WuXi AppTec.

    Enzyme-linked Immunosorbent Assay:

    Article Title: A small-molecule TNIK inhibitor targets fibrosis in preclinical and clinical models
    Article Snippet: Antibodies used were anti-human-fibronectin (Invitrogen, MA5-11981), anti-human E-cadherin (BD Biosciences, 610182), anti-human N-cadherin (D4R1H) (Cell Signaling, 13116S), anti-human phospho-SMAD2 (Ser465/467)/SMAD3 (Ser423/425) (D27F4) (Cell Signaling, 8828S), anti-human phospho-SMAD2 (Ser465/467) (Cell Signaling, 3108S), anti-human SMAD2/SMAD3 (D7G7) (Cell Signaling, 12470S), anti-human phospho-FAK (Tyr397) (D20B1) (Cell Signaling, 8556), anti-human FAK (Cell Signaling, 3285), anti-human TNIK (Cell Signaling, 32712), anti-human NF-κB p65 (D14E12) (Cell Signaling, 8242S), anti-human phospho-NF-κB p65 (Ser536) (93H1) (Cell Signaling, 3033S), anti-human β-catenin (Cell Signaling, 9562), anti-human α-tubulin (Abcam, ab18251), anti-human HDAC2 (Abcam, ab12169), anti-human histone 3 (Abcam, ab176842), anti-α-SMA antibodies (Cell Signaling, 19245; Abcam, ab5694; Abcam, ab32575), anti-GAPDH (Absin, 016D), anti-collagen I antibody (Abcam, ab34710; LSL), goat anti-mouse IgG (Abcam, ab205719), goat anti-rabbit IgG (Abcam, ab205718) and goat polyclonal antibodies conjugated to HRP (DAKO, K4003; Vector Lab, PI-1000). .. ELISA kits used were as follows: IL-1β (Sinobest Bio, YX-E01291M), IL-4 (Sinobest Bio, YX-E00064M), IL-6 (Sinobest Bio, YX-E00066M), TNF-α (Sinobest Bio, YX-E00104M), fibronectin (Takara, MK115), procollagen type I C peptide (Takara, MK101), rat hydroxyproline (KinesisDx, K11-0512), collagen (Abcam, ab222942). .. INS018_055 was provided by WuXi AppTec.

    Article Title: Effect of platelet-rich plasma on the biologic activity of the human rotator-cuff fibroblasts: A controlled in vitro study.
    Article Snippet: .. Subsequently, all samples were digested on a shaker at 60 ̊C for 3 h. Triplicates of samples were assessed immediately after digestion using the PicoGreen assay (Quant-iT PicoGreen assay, Molecular Probes, Eugene, OR).18 The procollagen content in the cell culture supernatant samples was assessed using a commercially available ELISA kit for the procollagen type I C-peptide (PIP EIA kit Takara Bio, Inc., Shiga, Japan).19 A procollagen assay, rather than a collagen assay was used for two reasons. ..

    Article Title: Low-Molecular-Weight Collagen Peptide Improves Skin Dehydration and Barrier Dysfunction in Human Dermal Fibrosis Cells and UVB-Exposed SKH-1 Hairless Mice.
    Article Snippet: .. The supernatants were analyzed using ELISA kits for MMP-1 (EHMMP1; Invitrogen, Miami, OK, USA) and Procollagen Type I C-Peptide (PIP; (MK101; Takara, Shiga, Japan)). .. Cell proliferation was assessed using the BrdU ELISA kit (Epoch, BioTek, Winooski, VT, USA).

    Article Title: A small-molecule TNIK inhibitor targets fibrosis in preclinical and clinical models.
    Article Snippet: Antibodies used were anti-human-fibronectin (Invitrogen, MA5-11981), anti-human E-cadherin (BD Biosciences, 610182), anti-human N-cadherin (D4R1H) (Cell Signaling, 13116S), anti-human phospho-SMAD2 (Ser465/467)/SMAD3 (Ser423/425) (D27F4) (Cell Signaling, 8828S), anti-human phospho-SMAD2 (Ser465/467) (Cell Signaling, 3108S), anti-human SMAD2/SMAD3 (D7G7) (Cell Signaling, 12470S), anti-human phospho-FAK (Tyr397) (D20B1) (Cell Signaling, 8556), anti-human FAK (Cell Signaling, 3285), anti-human TNIK (Cell Signaling, 32712), anti-human NF-κB p65 (D14E12) (Cell Signaling, 8242S), anti-human phospho-NF-κB p65 (Ser536) (93H1) (Cell Signaling, 3033S), anti-human β-catenin (Cell Signaling, 9562), anti-human α-tubulin (Abcam, ab18251), anti-human HDAC2 (Abcam, ab12169), anti-human histone 3 (Abcam, ab176842), anti-α-SMA antibodies (Cell Signaling, 19245; Abcam, ab5694; Abcam, ab32575), anti-GAPDH (Absin, 016D), anti-collagen I antibody (Abcam, ab34710; LSL), goat anti-mouse IgG (Abcam, ab205719), goat anti-rabbit IgG (Abcam, ab205718) and goat polyclonal antibodies conjugated to HRP (DAKO, K4003; Vector Lab, PI-1000). .. Enzyme-linked immunosorbent assay kits ELISA kits used were as follows: IL-1β (Sinobest Bio, YX-E01291M), IL-4 (Sinobest Bio, YX-E00064M), IL-6 (Sinobest Bio, YX-E00066M), TNF-α (Sinobest Bio, YX-E00104M), fibronectin (Takara, MK115), procollagen type I C peptide (Takara, MK101), rat hydroxyproline (KinesisDx, K110512), collagen (Abcam, ab222942). .. INS018_055 was provided by WuXi AppTec.

    Article Title: Low-Molecular-Weight Collagen Peptide Improves Skin Dehydration and Barrier Dysfunction in Human Dermal Fibrosis Cells and UVB-Exposed SKH-1 Hairless Mice
    Article Snippet: .. The supernatants were analyzed using ELISA kits for MMP-1 (EHMMP1; Invitrogen, Miami, OK, USA) and Procollagen Type I C-Peptide (PIP; (MK101; Takara, Shiga, Japan)). .. Cell proliferation was assessed using the BrdU ELISA kit (Epoch, BioTek, Winooski, VT, USA).

    Picogreen Assay:

    Article Title: Effect of platelet-rich plasma on the biologic activity of the human rotator-cuff fibroblasts: A controlled in vitro study.
    Article Snippet: .. Subsequently, all samples were digested on a shaker at 60 ̊C for 3 h. Triplicates of samples were assessed immediately after digestion using the PicoGreen assay (Quant-iT PicoGreen assay, Molecular Probes, Eugene, OR).18 The procollagen content in the cell culture supernatant samples was assessed using a commercially available ELISA kit for the procollagen type I C-peptide (PIP EIA kit Takara Bio, Inc., Shiga, Japan).19 A procollagen assay, rather than a collagen assay was used for two reasons. ..

    Cell Culture:

    Article Title: Effect of platelet-rich plasma on the biologic activity of the human rotator-cuff fibroblasts: A controlled in vitro study.
    Article Snippet: .. Subsequently, all samples were digested on a shaker at 60 ̊C for 3 h. Triplicates of samples were assessed immediately after digestion using the PicoGreen assay (Quant-iT PicoGreen assay, Molecular Probes, Eugene, OR).18 The procollagen content in the cell culture supernatant samples was assessed using a commercially available ELISA kit for the procollagen type I C-peptide (PIP EIA kit Takara Bio, Inc., Shiga, Japan).19 A procollagen assay, rather than a collagen assay was used for two reasons. ..

    Collagen Assay:

    Article Title: Effect of platelet-rich plasma on the biologic activity of the human rotator-cuff fibroblasts: A controlled in vitro study.
    Article Snippet: .. Subsequently, all samples were digested on a shaker at 60 ̊C for 3 h. Triplicates of samples were assessed immediately after digestion using the PicoGreen assay (Quant-iT PicoGreen assay, Molecular Probes, Eugene, OR).18 The procollagen content in the cell culture supernatant samples was assessed using a commercially available ELISA kit for the procollagen type I C-peptide (PIP EIA kit Takara Bio, Inc., Shiga, Japan).19 A procollagen assay, rather than a collagen assay was used for two reasons. ..



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